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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
23/08/2018 |
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Actualizado : |
23/08/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ABREO, E.; VALLE, D.; MUJICA, V.; ALTIER, N. |
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Afiliación : |
EDUARDO RAUL ABREO GIMENEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIANA VALLE LOPEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VALENTINA MUJICA TELIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Pathogenicity and virulence factors of Lysinibacillus xylanilyticus and Bacillus spp. towards Argyrotaenia sphaleropa larvae (Lepidoptera). |
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Complemento del título : |
Original contribution. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Journal of Applied Entomology, 2018, p.1-11. |
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DOI : |
10.1111/jen.12539 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 28 January 2018 / Accepted: 3 June 2018 / First published: 04 July 2018. |
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Contenido : |
Abstract
Argyrotaenia sphaleropa is a lepidopteran pest in deciduous fruit orchards in Uruguay. The primary control method of these pests is the use of chemical insecticides; however, alternative strategies, friendlier to the environment and compatible with a regional programme of integrated pest management, are definitely needed. Biological control with the use of local entomopathogenic microorganisms could be a sustainable solution. In this study, a biological screening was conducted to characterize spore‐forming bacilli for their capacity to kill larvae of this species. Bacillus thuringiensis, B. pumilus, B. aryabhattai, B. amyloliquefaciens and Lysinibacillus xylanilyticus were identified based on sequence analyses of 16S and partial GyrB gene. Lysinibacilus xylanilyticus showed the highest mortality towards first‐instar larvae fed with diet treated with spore suspensions. Specific virulence factors like proteinaceous parasporal inclusions were observed not only in isolates of B. thuringiensis but also in L. xylanilyticus. Ordering of isolates based on multivariate analysis of putative secondary virulence factors like biosurfactant and biofilm production, haemolytic activity and motility, separated the virulent isolate of L. xylanilyticus. This is the first report of L. xylanilyticus causing mortality in A. sphaleropa or any other member of Lepidoptera. A search for more virulent strains of this species is envisaged.
@© 2018 Blackwell Verlag GmbH |
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Palabras claves : |
16S; BIOFILM; CRY PROTEINS; HAEMOLYSIS; INTEGRATES PEST MANAGEMENT. |
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Thesagro : |
TORTRICIDAE. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02319naa a2200253 a 4500
001 1058944
005 2018-08-23
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1111/jen.12539$2DOI
100 1 $aABREO, E.
245 $aPathogenicity and virulence factors of Lysinibacillus xylanilyticus and Bacillus spp. towards Argyrotaenia sphaleropa larvae (Lepidoptera).$h[electronic resource]
260 $c2018
500 $aArticle history: Received: 28 January 2018 / Accepted: 3 June 2018 / First published: 04 July 2018.
520 $aAbstract Argyrotaenia sphaleropa is a lepidopteran pest in deciduous fruit orchards in Uruguay. The primary control method of these pests is the use of chemical insecticides; however, alternative strategies, friendlier to the environment and compatible with a regional programme of integrated pest management, are definitely needed. Biological control with the use of local entomopathogenic microorganisms could be a sustainable solution. In this study, a biological screening was conducted to characterize spore‐forming bacilli for their capacity to kill larvae of this species. Bacillus thuringiensis, B. pumilus, B. aryabhattai, B. amyloliquefaciens and Lysinibacillus xylanilyticus were identified based on sequence analyses of 16S and partial GyrB gene. Lysinibacilus xylanilyticus showed the highest mortality towards first‐instar larvae fed with diet treated with spore suspensions. Specific virulence factors like proteinaceous parasporal inclusions were observed not only in isolates of B. thuringiensis but also in L. xylanilyticus. Ordering of isolates based on multivariate analysis of putative secondary virulence factors like biosurfactant and biofilm production, haemolytic activity and motility, separated the virulent isolate of L. xylanilyticus. This is the first report of L. xylanilyticus causing mortality in A. sphaleropa or any other member of Lepidoptera. A search for more virulent strains of this species is envisaged. @© 2018 Blackwell Verlag GmbH
650 $aTORTRICIDAE
653 $a16S
653 $aBIOFILM
653 $aCRY PROTEINS
653 $aHAEMOLYSIS
653 $aINTEGRATES PEST MANAGEMENT
700 1 $aVALLE, D.
700 1 $aMUJICA, V.
700 1 $aALTIER, N.
773 $tJournal of Applied Entomology, 2018, p.1-11.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
06/06/2019 |
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Actualizado : |
07/06/2019 |
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Tipo de producción científica : |
Poster |
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Autor : |
BARAIBAR, S.; GERMAN, S.; PAREJA, L.; PEREYRA, S. |
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Afiliación : |
SILVINA BARAIBAR PEDERSEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUCIA PAREJA, Lucia Pareja Polo Agroalimentario y Agroindustrial, CENUR, Universidad de la Republica, Uruguay,; SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Fungicide strategies to manage wheat stem rust. |
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Complemento del título : |
838-P. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
In: Proceedings of the International Congress of Plant Pathology (ICPP) 2018: Plant Health in A Global Economy, Boston. |
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Idioma : |
Inglés |
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Contenido : |
Abstract: Stem rust (SR), caused by Puccinia graminis f.sp. tritici, is one of the most destructive diseases of wheat in the Southern Cone of America. The main factor contributing to this has been the increased use of susceptible cultivars. Medium-long term efforts pursue the development of adapted lines resistant to local and Ug99+races (potential risk). However, the optimization of short-term strategies comprising the use of foliar fungicides are needed to manage SR. Field trials were established in 2015 and 2016 in Uruguay to evaluate application moments of metconazole+epoxiconazole (first symptoms, ZGS65 and ZGS71) and nozzle types (CJ03®, TTJ60®) in SR susceptible (cv.B601) and intermediate (cv.B501) cultivars. Six commercial fungicides, including combinations of DMIs, QoIs and SDHIs and a non-treated and a complete treatment were evaluated in cv.B601 in both years. Fungicide application at disease onset significantly (p ? 0.05) reduced SR severity, AUDPC and increased grain yield, test weight and thousand-kernel weight as compared to the non-treated and applications at ZGS71. Epoxiconazole and metconazole were detected in harvested grain if applied at ZGS71. Yet, concentrations were below the maximum residue levels (CODEX and EU). Significant differences were found among fungicides for SR severity, if applied at first symptoms. These results suggest that fungicides can effectively reduce SR severity and increase grain yield in susceptible cultivars if applied at disease onset. MenosAbstract: Stem rust (SR), caused by Puccinia graminis f.sp. tritici, is one of the most destructive diseases of wheat in the Southern Cone of America. The main factor contributing to this has been the increased use of susceptible cultivars. Medium-long term efforts pursue the development of adapted lines resistant to local and Ug99+races (potential risk). However, the optimization of short-term strategies comprising the use of foliar fungicides are needed to manage SR. Field trials were established in 2015 and 2016 in Uruguay to evaluate application moments of metconazole+epoxiconazole (first symptoms, ZGS65 and ZGS71) and nozzle types (CJ03®, TTJ60®) in SR susceptible (cv.B601) and intermediate (cv.B501) cultivars. Six commercial fungicides, including combinations of DMIs, QoIs and SDHIs and a non-treated and a complete treatment were evaluated in cv.B601 in both years. Fungicide application at disease onset significantly (p ? 0.05) reduced SR severity, AUDPC and increased grain yield, test weight and thousand-kernel weight as compared to the non-treated and applications at ZGS71. Epoxiconazole and metconazole were detected in harvested grain if applied at ZGS71. Yet, concentrations were below the maximum residue levels (CODEX and EU). Significant differences were found among fungicides for SR severity, if applied at first symptoms. These results suggest that fungicides can effectively reduce SR severity and increase grain yield in susceptible cultivars if applied at diseas... Presentar Todo |
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Palabras claves : |
CHEMICAL CONTROL; FUNGUS; WHEAT. |
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Thesagro : |
TRIGO. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02088nam a2200193 a 4500
001 1059819
005 2019-06-07
008 2018 bl uuuu u00u1 u #d
100 1 $aBARAIBAR, S.
245 $aFungicide strategies to manage wheat stem rust.$h[electronic resource]
260 $aIn: Proceedings of the International Congress of Plant Pathology (ICPP) 2018: Plant Health in A Global Economy, Boston.$c2018
520 $aAbstract: Stem rust (SR), caused by Puccinia graminis f.sp. tritici, is one of the most destructive diseases of wheat in the Southern Cone of America. The main factor contributing to this has been the increased use of susceptible cultivars. Medium-long term efforts pursue the development of adapted lines resistant to local and Ug99+races (potential risk). However, the optimization of short-term strategies comprising the use of foliar fungicides are needed to manage SR. Field trials were established in 2015 and 2016 in Uruguay to evaluate application moments of metconazole+epoxiconazole (first symptoms, ZGS65 and ZGS71) and nozzle types (CJ03®, TTJ60®) in SR susceptible (cv.B601) and intermediate (cv.B501) cultivars. Six commercial fungicides, including combinations of DMIs, QoIs and SDHIs and a non-treated and a complete treatment were evaluated in cv.B601 in both years. Fungicide application at disease onset significantly (p ? 0.05) reduced SR severity, AUDPC and increased grain yield, test weight and thousand-kernel weight as compared to the non-treated and applications at ZGS71. Epoxiconazole and metconazole were detected in harvested grain if applied at ZGS71. Yet, concentrations were below the maximum residue levels (CODEX and EU). Significant differences were found among fungicides for SR severity, if applied at first symptoms. These results suggest that fungicides can effectively reduce SR severity and increase grain yield in susceptible cultivars if applied at disease onset.
650 $aTRIGO
653 $aCHEMICAL CONTROL
653 $aFUNGUS
653 $aWHEAT
700 1 $aGERMAN, S.
700 1 $aPAREJA, L.
700 1 $aPEREYRA, S.
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